Nr

Microsatellite list for biodiversity studies in chickens Nr.	Name	Chr.	Map	Primer sequence (5’ -> 3’) Forward Reverse	Annealing temperature	Genbank (Accession Number)	Multiplex	Allele range	Diversity studies
	ADL0268	1	M1	CTCCACCCCTCTCAGAACTA CAACTTCCCATCTACCTACT	60°C	G01688	X1	102-116	R1, R2, R4, R5
	MCW0206	2	M2	ACATCTAGAATTGACTGTTCAC CTTGACAGTGATGCATTAAATG	60°C	AF030579	X7	221-249	R2, R4, R5,
	LEI0166	3	M3	CTCCTGCCCTTAGCTACGCA TATCCCCTGGCTGGGAGTTT	60°C	X85531	X1	354-370	R1, R4
	MCW0295	4	M4	ATCACTACAGAACACCCTCTC TATGTATGCACGCAGATATCC	60°C	G32051	X3	88-106	R1,R2, R4, R5
	MCW0081	5	M6	GTTGCTGAGAGCCTGGTGCAG CCTGTATGTGGAATTACTTCTC	60°C	none	X2	112-135	R1,R2, R4, R5
	MCW0014	6	M7	TATTGGCTCTAGGAACTGTC GAAATGAAGGTAAGACTAGC	58°C	none	X4	164-182	R1,R2, R4, R5
	MCW0183	7	M8	ATCCCAGTGTCGAGTATCCGA TGAGATTTACTGGAGCCTGCC	58°C	G31974	X4	296-326	R1,R2, R4, R5
	ADL0278	8	M25	CCAGCAGTCTACCTTCCTAT TGTCATCCAAGAACAGTGTG	60°C	G01698	X1	114-126	R1,R2, R4, R5
	MCW0067	10	M9	GCACTACTGTGTGCTGCAGTTT GAGATGTAGTTGCCACATTCCGAC	60°C	G31945	X6	176-186	R1,R2, R4, R5
	MCW0104	13	M10	TAGCACAACTCAAGCTGTGAG AGACTTGCACAGCTGTGTACC	60°C	none	X5	190-234	R6
	MCW0123	14	M11	CCACTAGAAAAGAACATCCTC GGCTGATGTAAGAAGGGATGA	60°C	none	X5	76-100	R6
	MCW0330	17	M12	TGGACCTCATCAGTCTGACAG AATGTTCTCATAGAGTTCCTGC	60°C	G32085	X6	256-300	R1, R2, R6
	MCW0165	23	M13	CAGACATGCATGCCCAGATGA GATCCAGTCCTGCAGGCTGC	60°C	none	X5	114-118	R6
	MCW0069	E60C04W23	M14	GCACTCGAGAAAACTTCCTGCG ATTGCTTCAGCAAGCATGGGAGGA	60°C	none	X2	158-176	R2, R4, R5
	MCW0248	W29	M15	GTTGTTCAAAAGAAGATGCATG TTGCATTAACTGGGCACTTTC	60°C	G32016	X1	205-225	R1,R2, R4, R5

	MCW0111	1	M16	GCTCCATGTGAAGTGGTTTA ATGTCCACTTGTCAATGATG	60°C	L48909	X3	96-120	R1,R2, R4, R5
	MCW0020	1	M17	TCTTCTTTGACATGAATTGGCA GCAAGGAAGATTTTGTACAAAATC	60°C	none	X5	179-185	R6
	MCW0034	2	M18	TGCACGCACTTACATACTTAGAGA TGTCCTTCCAATTACATTCATGGG	60°C	none	X2	212-246	R4, R5
	LEI0234	2	M27	ATGCATCAGATTGGTATTCAA CGTGGCTGTGAACAAATATG	60°C	Z94837	X3	216-364	R4
	MCW0103	3	M19	AACTGCGTTGAGAGTGAATGC TTTCCTAACTGGATGCTTCTG	64°C	G31956	X7	266-270	R2, R4, R5
	MCW0222	3	M20	GCAGTTACATTGAAATGATTCC TTCTCAAAACACCTAGAAGAC	60°C	G31997	X2	220-226	R2, R4, R5
	MCW0016	3	M28	ATGGCGCAGAAGGCAAAGCGATAT TGGCTTCTGAAGCAGTTGCTATGG	60°C	none	X3	162-206	R6
	MCW0037	3	M24	ACCGGTGCCATCAATTACCTATTA GAAAGCTCACATGACACTGCGAAA	64°C	none	X3	154-160	R4, R5
	MCW0098	4	M21	GGCTGCTTTGTGCTCTTCTCG CGATGGTCGTAATTCTCACGT	60°C	none	X6	261-265	R2, R4, R5
	LEI0094	4	M5	GATCTCACCAGTATGAGCTGC TCTCACACTGTAACACAGTGC	60°C	X83246	X1	247-287	R4
	MCW0284	4	M30	CAGAGCTGGATTGGTGTCAAG GCCTTAGGAAAAACTCCTAAGG	60°C	G32043	X8	235-243	R4
	MCW0078	5	M22	CCACACGGAGAGGAGAAGGTCT TAGCATATGAGTGTACTGAGCTTC	60°C	none	X6	135-147	R1,R2, R4, R5
	LEI0192	6	M29	TGCCAGAGCTTCAGTCTGT GTCATTACTGTTATGTTTATTGC	60°C	Z83797	X8	244-370	R4
	ADL0112	10	M23	GGCTTAAGCTGACCCATTAT ATCTCAAATGTAATGCGTGC	58°C	G01725	X4	120-134	R1,R2, R4, R5
	MCW0216	13	M26	GGGTTTTACAGGATGGGACG AGTTTCACTCCCAGGGCTCG	60°C	AF030586	X1	139-149	R2, R4, R5

References:

Eding, H. (2002), Conservation of Genetic Diversity, Diss Wageningen University.
Van Marle Koster, E. and Nei, L.H. (2000), Genetic characterisation of native southern African chicken populations: Evaluation and selection of polymorphic microsatellite markers. South African Journal of Animal Science, 30: 1-6.
Wimmers, K., Ponsuksili, S., Hardge, T., Valle-Zerate, A., Marthur, P.K. and Horst, P. (2000) Genetic distinctness of African, Asian and South American local chickens. Animal Genetics. 31: 159-165.
Rosenberg et al. (2001), Empirical Evaluation of Genetic Clustering Methods Using Multilocus Genotypes From 20 Chicken Breeds, Genetics 159, 699-713. AVIANDIV project (https://aviandiv.fli.de>)
Hillel et al., (2003), Biodiversity of 52 chicken populations assessed by microsatellite typing of DNA pools, Genet. Sel. Evol. 35 533-557. AVIANDIV project
Weigend et al., unpubl. AVIANDIV project

Multiplexes:

Multiplex Master Mix Qiagen: ADL0278, ADL0268, LEI0094, MCW0248, MCW0216
Multiplex Master Mix Qiagen: MCW0081+MCW0034+MCW0069+MCW0222+MCW0295
Multiplex Master Mix Qiagen: MCW0111, MCW0037, MCW0016, LEI0166, LEI0234
Multiplex Master Mix Qiagen: MCW0183, ADL0112, MCW0014
Multiplex Master Mix Qiagen: MCW0165, MCW0020, MCW0123, MCW0104
Multiplex Master Mix Qiagen: MCW0078+MCW0067+MCW0330+ MCW0098
Hot Star Taq Master Mix Qiagen : MCW0206, MCW0103
no information

MCW0111

MCW0034